AdvanceBio Desalting-RP Columns
AdvanceBio Desalting-RP Columns
By providing simple, rapid reversed-phase removal of salt ions before MS detection, these cartridges allow MS detection with traditionally non-MS friendly techniques such as IEX, SEC, and affinity chromatography. Their use greatly reduces MS detector contamination, helping to minimize instrument maintenance and downtime.
- Intact & Subunit Analysis
Product Details
- Allow MS detection with traditionally non-MS friendly techniques such as IEX, SEC, and affinity chromatography.
- Save time by desalting your samples on-line.
- Concentrate samples prior to elution
- Reduce down time and maintenance by keeping your MS cleaner.
- Improve overall data confidence by improving the quality of your data.
- Suitable for large biomolecules with a large 1000 Å pore size
- pH stable from pH 2–13
- Compatible with all commonly used reverse phase mobile phases
Bio HPLC Columns eSelector Tools
Intuitive process to help you select the right chromatography column for your biopharmaceutical applications.
- Application Notes
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2D-LC as automated desalting tool for MSD analysis
Demonstion of the use of multiple heart-cutting two-dimensional liquid chromatography for automated desalting of glucagon from an MS incompatible USP method.
- Application Notes
- English
- 25 Oct 2024
- 1.21 MB
Efficient Monoclonal Antibody and Antibody Drug Conjugate Desalting Prior to Mass Spectrometry using AdvanceBio Desalting-RP Cartridges
Presents the use of AdvanceBio Desalting-RP cartridges to pretreat mAb and ADC samples before MS analysis.
- Application Notes
- English
- 01 Nov 2018
- 2.11 MB
Cell Clone Selection Using the Agilent Bio-Monolith Protein A Column and LC/MS
An Agilent Bio-Monolith Protein A column determined recombinant mAb titer and was used to enrich µg amounts of antibody for further structural characterization by MS.
- Application Notes
- English
- 22 Nov 2017
- 264.06 KB
Agilent AdvanceBio Desalting-RP Cartridges for Online Desalting in 2D-LC/MS mAb Analysis
Use of a desalting cartridge in the second dimension to desalt and characterize monoclonal antibodies (mAbs) using a 2D-LC/MS setup.
- Application Notes
- English
- 16 Sep 2016
- 749.05 KB
Application of AdvanceBio Desalting - RP Cartridges for LC/MS Analysis of mAbs
AdvanceBio Desalting - RP cartridges used for effective desalting of LC-separated monoclonal antibodies (mAbs) before characterization by mass spectrometry
- Application Notes
- English
- 03 Aug 2016
- 889.38 KB
Characterize Fab and Fc Fragments by Cation-exchange Chromatography
Agilent Bio Mab WCX separates charged variants of papain-digest monoclonal antibody, following by MS characterization of the charged variants with online desalting.
- Application Notes
- English
- 04 Dec 2014
- 279.37 KB
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Bio LC Column User Guides
User guides contain helpful information to ensure the best performance from your column, such as operating parameters, cleaning and storage guidelines, usage tips, and suggested starting conditions.
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AdvanceBio Webinars Series
Advance the LC Characterization of Biotherapeutics, a webinar presented by industry experts
Biopharma Webinars
A Series of Live Webinars discussing Workflows in the Biopharmaceutical disciplines. A Combination of Agilent scientists and Industry speakers contribute
Agilent Wins Multiple 2022 Scientists' Choice Awards
Agilent received two 2022 Scientists’ Choice Awards for Drug Discovery & Development, Virtual Event of the Year and Innovative Content of the Year, at the SLAS2022 International Conference & Exhibition on February 7th, 2022. The Scientists’ Choice Awards recognize the world’s most innovative and effective analytical science technologies and communications.
- Agilent Wins Multiple 2022 Scientists' Choice Awards
- 15 Feb 2022
Agilent Launches New AdvanceBio Spin Columns For Desalting and Buffer Exchange
AdvanceBio Spin Columns address the need for efficient sample preparation of proteins or oligonucleotides under aqueous conditions, preserving native structure with suitable ionic strength and pH. Users can easily and quickly remove excess salts or undesired small molecules from their sample matrix or exchange the sample matrix between different buffer conditions. These gel-filtration-based spin columns are available in analytical and semipreparative scale columns for single samples, as well as 96-sample plates for high-throughput settings.
- 01 Apr 2024