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HPLC Size Exclusion Chromatography (SEC) Columns and Standards

Agilent offers size exclusion chromatography (SEC) HPLC columns in a wide variety of pore sizes to address every aggregate or fragment analysis challenge. AdvanceBio SEC columns featuring unique hydrophilic bonding for minimal secondary interactions are available in 2.7 µm and 1.9 µm particle sizes, for high throughput, high resolution separations.

Aggregate & Fragment Analysis

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Bio HPLC Columns eSelector Tools

Intuitive process to help you select the right chromatography column for your biopharmaceutical applications.

Preparative HPLC Scaling Calculator

Scale between analytical and prep methods; calculate column capacity, # of injections, run time & mobile phase use.

Oligonucleotide Virtual Lab

Explore proven methods and resources to help advance analysis for the broad range of oligonucleotide workflows.

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N-Glycan Analysis: Better Together

Push your Glycoprotein analysis to new levels of performance.

Glycan Standard Structures

Catalog of glycan standards and libraries, complete with quick links to easily get more details for each online.

Agilent Biocolumns - Critical Quality Attributes - Application Compendium

Critical Quality Attributes application compendium

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Featured News

Agilent Launches New AdvanceBio Spin Columns For Desalting and Buffer Exchange

AdvanceBio Spin Columns address the need for efficient sample preparation of proteins or oligonucleotides under aqueous conditions, preserving native structure with suitable ionic strength and pH. Users can easily and quickly remove excess salts or undesired small molecules from their sample matrix or exchange the sample matrix between different buffer conditions. These gel-filtration-based spin columns are available in analytical and semipreparative scale columns for single samples, as well as 96-sample plates for high-throughput settings.

Agilent Launches New Tool To Identify InstantPC- or 2-AB-labeled N-glycan Peaks

The free Glycan Peak Assignment Tool was developed to provide additional confidence in identifying InstantPC- or 2-AB-labeled N-glycan peaks by predicting retention times of over 100 glycans. The new tool does not require users to upload sensitive data from N-glycan samples.

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