The Human 14 Multiple Affinity Removal Column is designed to chromatographically remove fourteen interfering high-abundant proteins from human plasma samples. Removal of these proteins improves subsequent LC/MS and electrophoretic analysis of the sample by effectively expanding the dynamic range of the analysis. This enables easier isolation and identification of proteins in the serum.
This product is not available for purchase by the general public.
Product Details
Features
Eliminates approximately 94% of total protein (albumin, IgG, antitrypsin, IgA, transferrin, haptoglobin, fibrinogen, alpha2-macroglobulin, alpha1-acid glycoprotein, IgM, apolipoprotein AI, apolipoprotein AII, complement C3 and transthyretin) when used with the Multiple Affinity Removal System
Three column dimensions include 4.6 x 50 mm, 4.6 x 100 mm, 10 x 100 mm - custom sizes also available
Plasma injection volume ranges between 20-40uL per run for the 4.6mm id, and 250–300 µL for the 10 mm id column
Optimize sample recovery by utilizing our rapid, single-injection protocols and optimized buffers
Designed for over 200 injections
Process the most plasma, over the column lifetime, up to 4000uL, for the 4.6mm id columns and 60,000 µL for the 10 mm id column
Optimized for use with human physiological samples (not recommended for use with mouse serum)
Compatible with most LC or HPLC pumps or instruments
A guide to find the right column for your characterization workflow. It includes advice and tips on method development, solvent choice, mobile phase modification, optimization, example separations, as well as ordering information.