Cell-free DNA ScreenTape analysis provides fast, automated, and reliable electrophoresis of input cell-free DNA (cfDNA) samples. The Cell-free DNA ScreenTape assay for TapeStation systems provides accurate sizing and quantification of cfDNA, as well as DNA quality assessment with the objective %cfDNA quality metric. The %cfDNA score automatically provides the percentage of cfDNA in the sample of interest compared to genomic DNA contamination - essential for cell-free DNA integrity analysis in Next Generation Sequencing (NGS) library quality control (QC) and for generating reliable NGS results.
The assay is compatible with the Agilent 4150 and 4200 TapeStation instruments for automated electrophoresis.
For Research Use Only. Not for use in diagnostic procedures.
The Sample Processing Lab (SPL) collaborated with the Genomics and Proteomics Core Facility (GPCF), both part of the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) to track 13 cfDNA samples with the TapeStation system from initial QC with the %cfDNA metric, through the NGS workflow, to the final sequencing results.
In this technical overview, detection of contaminating high molecular weight DNA is discussed, comparing analyses between Bioanalyzer and TapeStation systems.
Cell-free DNA has become an important source for potential new biomarkers. Yet, it represents a challenging sample type due to its low yield and complex fragment size distribution. Controlling and monitoring pre-analytical parameters, including cfDNA extraction, is crucial for experimental success A new automated electrophoresis assay was used to compare nine different kits tailored for cfDNA extraction.
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