Mammalian Transfection Kit - Details & Specifications
Our mammalian transfection kit contains the necessary reagents and protocols for efficient, rapid and simplified DNA transfection of mammalian cells. The kit has been optimized for thirty transfections in 100-mm tissue culture dishes using a modified CaPO4 protocol for the production of stable transfectants, and thirty transfections using a DEAE-dextran protocol for transient transfections.
Solutions #1 and #2 are used in the modified CaPO4 precipitation protocol. This method is most frequently used to produce stable transfectants. The modified CaPO4 protocol may also be useful in performing transient transfection assays in cases where it may not be appropriate to use the DEAE-dextran method.
Solution #3 is used in the DEAE-dextran protocol. This method is simple, quick and efficient, and is generally used for transient transfections.
For Research Use Only. Not for use in diagnostic procedures