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The Agilent Advantage – Measure Immune Cell Activation in Minutes

RAPID, REAL-TIME DETECTION
OF MACROPHAGE ACTIVATION
Agilent Seahorse XFp Analyzer
RAPID, REAL-TIME DETECTION
OF T CELL ACTIVATION
Agilent Seahorse XFp Analyzer
Cellular energy metabolism is now understood to influence many immune cell pathways as both an indicator and controller of immune cell function and lineage commitment. This concept is clearly illustrated in the activation of naive T cells to stimulate proliferation and macrophages to stimulate pro-inflammatory signaling. Expand these sections below to learn more about accelerating your immune cell research with XF technology.
Immune Cell Activation Application Notes & Technical Information

+ MACROPHAGE ACTIVATION Real-time analysis of in situ activated macrophages

+ T CELL ACTIVATION Real-time analysis of in situ activated T cells

Activate
  • Real-time discrimination of immune cell activation responses.
Modulate
  • Introduce up to 4 modulators per well with programmable drug injection ports to elucidate immune cell response to activation kinetics.
Investigate
  • Explore the metabolism driving immune cell response through assays providing metabolic phenotype, bioenergetic pathway, and fuel/substrate preference.
Adapted from Hongbo Chi, Nat Rev Immunol.;
12(5): 325-338, 2012
The Cell Lineage and Immunometabolism Link
Advances in the field of immunology show cellular metabolism as a driver and regulator of immune cell function and differentiation. T cell and macrophage activation are correlated with metabolic shifts toward glycolysis, activating energy promoting pathways required for high rates of proliferation or signaling.
Modulate immune cell activation
with the use of programmable drug injection ports
Agilent Seahorse XF sensor cartridges incorporate integrated injection ports that sequentially deliver up to 4 compounds, in addition to immunogenic activation beads, allowing dose response, agonist or antagonist response, or pathway perturbation analysis of each sample.
Dive deeper with standardized cell metabolism assays and powerful software
In addition to T cell and macrophage activation, Seahorse metabolic assay kits are available for measuring mitochondrial respiration, glycolysis, and fatty acid oxidation in cells. Seahorse XF kits and reagents are pre-calibrated, pre-tested, and are coupled with powerful report generator software, providing functional metabolic data from live immune cells.
Metabolic Phenotype
Rapidly determines the energy phenotype/fingerprint of cells and reveals their metabolic potential
Mitochondrial Profiling
Provides a complete mitochondrial profile of your immune cells using the mitochondrial stress test kit
Glycolysis Profiling
Provides a complete glycolytic profile of your immune cells using the glycolytic stress test kit
Don’t wait to accelerate your research! Learn more about XF Technology.
For Research Use Only. Not for use in diagnostic procedures.