AdvanceBio Gly-X Technology

AdvanceBio Gly-X technology is a new generation of N-glycan sample preparation. Be ready for sample analysis faster with rapid (5-minute) enzymatic release of N-glycans and labeling with InstantDyes including the brightest in class InstantPC, or traditional reductive amination dyes. Innovative in-solution workflows combined with plate-based rapid cleanup enables sample prep in as little as 1 hour. AdvanceBio Gly-X Increases analytical sensitivity with a variety of dye options, while increasing sample throughput.

N-Glycan Sample Preparation Kits

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Features

Three dyes are currently available for the Gly-X platform:
InstantPC: a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.
2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2 hours.
InstantAB: ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Superseded by InstantPC, which has higher fluorescence and MS signal (see Agilent publication 5994-0942EN). Sample prep time: ~1 hour.

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